Figure 1: YMC Accura BioPro IEX columns feature a bioinert coating of the column body and frits.
Figure 2: YMC Accura BioPro IEX columns provide high recovery and very good peak shapes from the first injection making them ideal for the analysis of oligonucleotides (here: 21mer RNA) with reproducible results.
Figure 3: Smaller column IDs that allow lower flow rates and volatile mobile phases are necessities for coupling to MS. Such high sensitivity analyses of mAbs (here: trastuzumab) are ideally performed using YMC Accura BioPro IEX columns.

New application note: AEX-MS - a powerful tool for charge heterogeneity characterisation of an IgG4-based mAb

Robust bioinert columns for a new level of recovery: YMC Accura BioPro IEX columns

Apr 22 2024

Ion exchange chromatography (IEX) is widely used for Bio QC methods, especially for charged variant analyses. Sensitive substances like oligonucleotides tend to interact with column hardware, even with quite inert IEX standard hardware made of PEEK. Adsorption of the analytes on the surfaces lead to peak tailing, carryover, and reduced recovery.

Special challenges

Sensitive substances like oligonucleotides tend to interact with column hardware, even with quite inert IEX standard hardware made of PEEK. In addition, diverse peptides and proteins are prone to interaction with the column body, too. Adsorption of the analytes on the surfaces lead to peak tailing, carry-over, and reduced recovery. In the past, time and solvent-intensive preconditioning was the only measure. Fortunately, suitable bioinert column hardware makes it much easier nowadays.

Bioinert column hardware as solution

YMC meets this special challenge with the recently launched YMC Accura columns. They feature a bioinert surface coating of the column body and frits. The robust bioinert coating used on YMC Accura hardware is 130 to 320-fold thicker compared to similar hardware concepts. Permanent inertness against sensitive substances is ensured. Therefore, YMC Accura columns can even be used for the analysis of sensitive substances without pre-conditioning.

Ideal stationary phases

The non-porous hydrophilic polymer beads of the BioPro IEX stationary phases enable exceptionally high efficiencies and high throughput analyses. Even small sample variations can be easily separated and detected. The superior lot-to-lot reproducibility guarantees highly reliable results. BioPro IEX SF with its strong cation exchanger functionality is particularly ideal for analysing charge variants of monoclonal antibodies (mAbs) or other proteins. The strong anion exchanger BioPro IEX QF is the first choice for high-resolution analysis of oligonucleotides of different lengths as well as mAbs with lower pI.

Benefits from using YMC Accura BioPro IEX columns

The combination of the bioinert coated column hardware and the high efficiency stationary phases of YMC Accura BioPro IEX columns provide excellent peak shapes for sensitive compounds. They ensure high recovery from the very first injection and prevent carry-over. YMC Accura BioPro IEX columns are not only very inert, but are also available with smaller column IDs, making them ideal for use in native IEX-MS analysis.