• New application note: AEX-MS - a powerful tool for charge heterogeneity characterisation of an IgG4-based mAb

Columns

New application note: AEX-MS - a powerful tool for charge heterogeneity characterisation of an IgG4-based mAb

The efficacy and safety of mAbs is ensured by tightly controlled critical quality attributes (CQA) such as the charge heterogeneity. To monitor the CQAs of mAbs, sensitive and reliable analytical methods are essential. This application note demonstrates the successful charge heterogeneity analysis of an IgG4-based mAb with a pI of 6.6 using a strong anion exchange column, BioPro IEX QF, coupled to mass spectrometry (MS) detection. PNGase F-mediated deglycosylation enhanced the resolution of glycosylated variants, allowing for precise monitoring of Fc CQAs.

Download the application note.

More information online


Digital Edition

LMUK 49.7 Nov 2024

November 2024

News - Research & Events News   - News & Views Articles - They’re burning the labs... Spotlight Features - Incubators, Freezers & Cooling Equipment - Pumps, Valves & Liquid Hand...

View all digital editions

Events

Turkchem

Nov 27 2024 Istanbul, Turkey

Smart Factory Expo 2025

Jan 22 2025 Tokyo, Japan

Instrumentation Live

Jan 22 2025 Birmingham, UK

SLAS 2025

Jan 25 2025 San Diego, CA, USA

Arab Health

Jan 27 2025 Dubai, UAE

View all events