New 1 L single-use bioreactor for cell culture and microbial applications

At this year’s Biotechnica, Eppendorf presented the newest members of its single-use bioreactor family BioBLU for the first time. With the new single-use bioreactor BioBLU 1c for cell culture and BioBLU 1f for microbial applications Eppendorf further extends its portfolio of rigid wall single-use bioreactors. These 1L vessels are bridging the gap between the BioBLU 0.3 c/f mini single-use bioreactors and the larger vessels of the BioBLU family. Users in cell culture, who want to combine the advantages of single-use technology with the proven qualities of stirred-tank bioreactor design can now benefit from an unmatched portfolio of rigid wall stirred tank single-use bioreactors with working volumes from 100mL – 40L.

The BioBLU 1c/f single-use bioreactors have been specifically developed for the use with DASGIP® Parallel Bioreactor Systems. They are perfectly suited to manage the various challenges in bioprocess development. With DASGIP systems 4, 8 and more bioreactors can be operated in parallel, in both the cultivation of animal and human cells as well as for microbial applications where bacteria and yeasts users profit by parallel processing. Effective process planning and automation, design of experiment (DoE) and comprehensive bioprocess information management give an extra plus for time and cost effective bioprocessing.   

The fully instrumented rigid wall BioBLU 1c/f single-use bioreactors can be operated with working volumes of 320mL – 1.25L in cell culture and 250mL – 1.25L in microbiology. All critical process parameters such as temperature, pH and dissolved oxygen can be measured and controlled via industry standard sensors. Integrated dip-tubes allow for controlled addition of liquids, sampling and controlled submerged and/or headspace gassing. The BioBLU 1c comes with two 3x45° pitched blade impellers, the BioBLU 1f with three 6-blade Rushton-type impellers. The specifically developed encapsulated, magnet-coupled stirrer supports sterile and powerful agitation with up to 600 rpm in cell culture and 1600 rpm for microbial applications.

The innovative liquid-free Peltier condenser provides optimal cool down of exhaust. The BioBLU 1f additionally carries four cooling-baffles which support precise temperature control even with modern high cell density processes.

All wetted materials of the BioBLU 1c/f single-use bioreactors are USP Class VI certified and conform to United States Food and Drug Administration requirements.