Laboratory Products
Complete methylome analysis from only 50 ng of genomic DNA
Nov 14 2013
Cambio, the Cambridge, UK-based supplier of world-class molecular biology
reagents and consumables, has launched the EpiGnomeTM Methyl-Seq Kit in the UK.
EpiGnome, developed by US biotech firm Epicentre, an Illumina company,
produces whole genome bisulfite sequencing libraries from as little as 50 ng
of genomic DNA. The kit uses a unique 'post-bisulfite conversion' library
construction method that yields high diversity libraries with uniform
coverage.
The process of bisulfite treatment denatures genomic DNA (gDNA) into
single-stranded DNA (ssDNA). EpiGnome converts all the ssDNA into an
IlluminaR sequencing library, eliminating the sample loss associated with
other methods.
The elimination of sample loss using EpiGnome means that as little as 50 ng
of total DNA is needed to detect methylation. Uniform coverage is clearly
seen at this scale (Figure 1).
EpiGnome has the sensitivity to detect methylation changes with only 50 ng
of input gDNA. When 50 ng of lymphoblastoid gDNA was treated with bisulfite
and converted into a sequencing library using EpiGnome (first example), the
CpG methylation pattern across regions of chromosome 1 highlighted areas of high CpG methylation (red) and low CpG methylation (blue). Similarly, when 50 ng of a hypermethylated HeLa sample was treated with bisulfite and
converted into a sequencing library using EpiGnome (second example), most
reads showed methylation (red), and very few reads were not methylated
(blue).
EpiGnome offers uniform CpG, CHG and CHH coverage. The method does not require fragmentation by Covaris ultrasonication nor methylated adaptors, as required by other bisulfite sequencing library prep methods. In addition to
eliminating sample loss, the EpiGnome method takes only 5 hours, a fraction
of the time taken by alternative methods.
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