Microscopy & Microtechniques
Only Complete System for the Visualization of Dynamic Processes at the Cell Membrane Delivers Three World Firsts.
Nov 06 2006
The new microscope is the first to offer the combination of TIRF and transmitted-light contrasting techniques, such as DIC and brightfield, which enables sequential recording of two image pairs per second. By selectively exciting cellular fluorophores adsorbed, adhered, or bound to the surface and combining it with conventional epi-fluorescence, researchers can relate surface effects to internal cellular structures.
The Laser TIRF is also the first TIRF system to offer rapid laser line changes, thanks to a fast, double filter wheel and includes AxioVision software that permits on-screen alignment without compromising laser safety. The new optical system boasts a multi-line argon laser with wavelengths of 458, 488 and 514 nm, ideal for living dyes such as CFP, GFP, YFP and mRFP. Its unique geometry ensures that TIRF is maintained while switching wavelengths, a problem with other TIRF systems.
The Laser TIRF imaging system is based on the Zeiss Axiovert 200 research microscope. It is ideal for applications that include the selective visualisation of cell/substrate contact regions, tracking secretion in living cells, measuring the binding rates of cell surface receptors, the examination of submicroscopic morphology, and single molecule experiments on molecular motors.
Digital Edition
ILM 49.5 July
July 2024
Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...
View all digital editions
Events
Jul 28 2024 San Diego, CA USA
Jul 30 2024 Jakarta, Indonesia
Jul 31 2024 Chengdu, China
ACS National Meeting - Fall 2024
Aug 18 2024 Denver, CO, USA
Aug 25 2024 Copenhagen, Denmark