Microscopy & Microtechniques
Elispot Software Speeds Up Vaccine Research
Apr 25 2008
The KS Elispot compact based around the Zeiss Stemi 2000 C stereomicroscopes will read and evaluate the results of a 96-well microplate in less than four minutes and is ideally suited to the routine laboratory. For the research laboratory, the Axio Imager based KS Elispot will distinguish spots as small as two microns and, combined with the AxioCam camera and suitable filters, enables fluorescence Elispot evaluation.
Whether in normal illumination or fluorescence mode, the new software
allows users to set up convenient shortcuts to established operating
parameters rather than searching through a series of drop-down menus. For instance, opening the configuration panel and setting the exposure time of the camera is a single mouse click. A neutral frame can be added to single well images to improve spot recognition at the
periphery. Errors are also reduced with a facility that stops the AxioCam camera imaging in the reduced-resolution binning mode
and re-evaluation of stored images can also be blocked to prevent unintentional or unauthorised changes. Finally, KS Elispot software has been designed to integrate seamlessly with the Zeiss imaging suite, AxioVision 4.6. Elispot (Enzyme-linked immunosorbent spot) is a highly sensitive version of the Elisa immunoassay used routinely for monitoring immune responses in humans and animals.
Originally developed to enumerate B cells secreting antigen-specific antibodies, Elispot has been adapted for various tasks, especially the identification and enumeration of cytokineproducing cells at the single cell level where they allow visualisation of the secretory product of individual activated or responding cells. Because each spot represents a single reactive cell, Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells)information. Limits of detection are below 1/100,000 rendering the assay uniquely useful for monitoring antigen-specific responses, applicable to a wide range of areas of immunology research, including cancer, transplantation, infectious disease, and vaccine development.
Digital Edition
ILM 49.5 July
July 2024
Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...
View all digital editions
Events
Jul 28 2024 San Diego, CA USA
Jul 30 2024 Jakarta, Indonesia
Jul 31 2024 Chengdu, China
ACS National Meeting - Fall 2024
Aug 18 2024 Denver, CO, USA
Aug 25 2024 Copenhagen, Denmark