Tecan’s Infinite® 200 PRO offers a complete solution for cell-based measurements

The Gas Control Module (GCM™) for the Infinite 200 PRO is a breakthrough for cell-based assays, offering precise, simultaneous regulation of oxygen and carbon dioxide levels within the reader chamber for the first time.

Variations in environmental conditions can lead to inconsistent and unreliable data for cell-based optical studies, due to changes in the pH and color of the media during incubation. The GCM provides a more stable culture environment over time, making it ideally suited to in vitro investigation of eukaryotic cell lines and allowing the duration of cell­-based experiments to be extended without adversely affecting results.

Launched in 2010, the patent pending GCM for the Infinite 200 PRO multimode reader was a breakthrough for cell­based experiments, and has since been further enhanced to allow simultaneous control of O₂ and CO₂ concentrations, offering rigorous environmental control for a wide range of cell biology applications.

The enhanced GCM uses improved sensor technology to increase measurement accuracy and minimize cross-interference for oxygen measurements. Combined with independent gas inlets, this offers precise, independent control of both O₂ and CO₂ levels within the reader, helping to further stabilize culture conditions for more consistent cell growth. The greater sensitivity and precision offered by this set-up also allows the instrument to automatically compensate for variations in the atmospheric partial pressure of CO₂, using the module’s unique altitude correction function, which is accessed via an intuitive new user interface.

Advanced process security provides rapid detection of changes in gas pressure or flow to identify if the target concentration will not be reached or deviates significantly during incubation, with both audible and visible warnings to alert the user. The Infinite 200 PRO’s i-­control™ software generates a log of gas concentrations throughout the experiment, and this data will also be added to the results file alongside each data point, ensuring comprehensive process monitoring and maximum data integrity.

These advanced features ensure greater biological relevance for a wide range of studies, extending the experimental window for microplate-based investigations of anaerobic or facultative anaerobic bacteria, and allowing close replication of hypoxic or physiological conditions for eukaryotic cells. Together with the Infinite 200 PRO’s cell biology oriented functions – including Optimal Read (OR), linear and orbital shaking, advanced temperature control and automated z-focusing – this comprehensive solution sets new standards for cell-based assays.

To find out more on Tecan’s Infinite 200 PRO and Gas Control Module, visit www.tecan.com/gcm or click here.