SENSOLUX®: Monitoring Process Parameters Using an Optical Sensor System

The SENSOLUX® stand-alone version is a shaker platform equipped with an optical sensor system for monitoring pH and dissolved oxygen (DO) saturation during cultivation of animal cells. This first member of the SENSOLUX® family is an attractive tool for obtaining conclusive results in the early process development phase, e.g., for advanced clone screening and media optimization. Used in combination with the single-use Erlenmeyer flasks SENSOLUX® EF, the shaker platform facilitates easy, non-invasive, reliable and highly informative online measurement of these crucial process parameters in incubation shakers. For this purpose, SENSOLUX® EF are equipped with two pre-calibrated sensor patches. In total, nine measurement points for both pH and DO are integrated into the shaker tray that has a compact footprint of 420 mm x 420 mm and is compatible with a broad range of standard incubation shakers, including the CERTOMAT® shaker range from Sartorius Stedim Biotech. SENSOLUX® EF Erlenmeyer flasks are available in a choice of four sizes: 125 ml, 250 ml, 500 ml and 1,000 ml. The flasks are supplied sterile, individually packaged and ready-to-use. Special clamps ensure exact positioning of the flasks on the shaker tray and, therefore, precise measurement. The SENSOLUX® system comes with dedicated software, which enables monitoring and visualization of the parameters measured in each flask. Sartorius AG.