Laboratory Products
Automated Pipetting Technology Helps LGC Make the Most of Real-time PCR
Dec 10 2007
Founded over 150 years ago as the Laboratory of the Government Chemist, LGC has been an independent, privately owned company for the past ten years. Services provided by LGCâs state-of-the-art facilities include chemical, biochemical and forensic analysis, DNA testing and genetic screening. LGCâs BioAnalysis team uses real-time PCR techniques to analyse DNA extracted from a variety of sources, such as bacterial cells and foods.
Dr Gavin Nixon is a senior researcher in the BioAnalysis team at LGC: âA recent DTI-funded contract research project in the personal care/clinical sector required rapid characterisation of bacterial populations present within clinical samples. This large-scale study involved detecting indicator microbes and generating high quality quantitative data, in order to make comparisons between real-time PCR and standard microbiological techniques. Manual plate set-up of a 96-well format was not an option because of the limited time available for the study and the need to reduce reagent costs, save staff time and maintain experimental design flexibility. After a comprehensive evaluation of various different liquid handling systems, the team finally selected Eppendorfâs epMotion 5075 LH, based on its flexibility, pipetting accuracy at extremely small volumes and very user-friendly software.
âWith single or multichannel tools and accurate pipetting of volumes as small as one microlitre, the epMotion gives us the flexibility to reduce sample volumes and the associated costs. The newly released epBlue software is very simple and intuitive to use, enabling us to upload new methods very easily and even to manage the process remotely. Significantly, the advantages of automation are combined with increased reproducibility of results and high quality data output â which is vital for the services LGC delivers.â
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