Laboratory Products
New Kits Take Ribosomal RNA to Virtually Zero
Feb 08 2012
Available exclusively in the UK from Cambio, Epicentre’s species-specific Ribo-Zero kits remove virtually all small RNA subunits from total RNA preparations in a single extraction. The latest product developments - Ribo-Zero rRNA Removal Kit (Meta-Bacteria) and Ribo-Zero Gold Kit (Human/Mouse/Rat) - remove cytoplasmic and mitochondrial RNA, setting even higher standards, and delivering enhanced profiles of RNA-seq libraries.
Addressing the increasing use of RNA sequencing techniques, Ribo-Zero rRNA Removal Kits remove more ribosomal RNA from intact and partially degraded RNA than any other method. Applicable for use with a variety of eukaryotic species, Epicentre’s Ribo-Zero technology achieves outstanding results in a time saving single-pass process. For example, the popular kit for Human/Mouse/Rat RNA removes more than 99.9% of 28S, 18S and 5.8S rRNA. The latest Ribo-Zero Gold Kit (Human/Mouse/Rat) is an enhanced version giving superior results, and a lowinput version of the Human/Mouse/Rat kit is ideal for removing rRNA from formalin-fixed paraffinembedded
(FFPE) RNA samples.
Ribo-Zero kits are also available from Cambio for a wide spectrum of gram-positive and gram-negative bacteria, as well as for plant leaf and plant seed/root material. The new Ribo-Zero rRNA Removal Kit (Meta-Bacteria) removes more than 98% of 5S rRNA, in addition to >98% of 23S and 16S rRNAs, from RNA preparations of both pure and mixed populations of gram-negative and gram-positive bacteria. Ribo-Zero kits are compatible with ScriptSeq
preparation kits and their ability to remove virtually all cytoplasmic and mitochondrial RNA improves the success of subsequent Next-Gen RNA Sequencing, random-primed cDNA synthesis and other applications.
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