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Real Time PCR analysis of MethylCollector™ Ultra Analysis of the unbound and eluted fractions by real time PCR shows the enrichment of methylated DNA for both the Xist and NY-ESO loci, while the unmethylated APC locus is not detected in the eluted fraction
Methylation Analysis from as little as 1 ng (170 cells) DNA. The MethylCollector™ Ultra Kit has been shown to specifically detect CpG-methylated DNA from as few as 170 cells (~1 ng DNA). Varying amounts of Mse I digested human, male genomic DNA was tested in the MethylCollector™ Ultra Kit. The unbound and eluted fractions were collected and analyzed using real time PCR with the included Xist PCR primer mix. Methylated DNA was recovered from as little as 500 pg, but enrichment of methylated DNA is seen with 1 ng or more of input material.

DNA / RNA

Mar 02 2010

DNA / RNA

AirClean® Systems’ microprocessor-controlled Combination PCR Workstations reduce the risk of sample contamination during DNA/RNA amplification, creating a...

AirClean® Systems combination PCR workstation

DNA / RNA

Alpha Laboratories Ltd. is pleased to announce the launch of Amplify Rigid PCR Plates, designed to set a new standard in laboratory efficiency and reliabil...

PCR plates for robotic automation applications

DNA / RNA

Ingenza has secured innovation funding to adapt its advanced machine learning platform for gene design. The initiative aims to precisely control recombinant...

Gene design algorithm to aid recombinant protein production in yeast

DNA / RNA

In collaboration with ProtonDx, Alpha Laboratories introduces SmartLid™, an innovative magnetic key and lid system revolutionising the nucleic acid extract...

Efficient and eco-friendly nucleic acid extraction

Digital Edition

LMUK 49.7 Nov 2024

November 2024

News - Research & Events News - News & Views Articles - They’re burning the labs... Spotlight Features - Incubators, Freezers & Cooling Equipment - Pumps, Valves & Liquid Hand...