Extra stability, alternative selectivity phases for increased choice in UHPLC/HPLC method development

Two new “Extra Stability”, “Alternative Selectivity” Ultra-Inert phases are now available from Advanced Chromatography Technologies Limited. ACE C18-Amide and ACE CN-ES incorporate the latest developments in UHPLC/HPLC stationary phase design and provide chromatographers with more choices for alternative selectivity without compromising stability or robustness. ACE C18-Amide and ACE CN-ES complement and enhance the already existing offering of “Extra Stablity” and “Alternative Selectivity” phases from Advanced Chromatography Technologies which includes the ACE C18-AR and ACE C18-PFP phases.

ACE C18-Amide is a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. The special design of the ACE C18-Amide ligand incorporates an extended alkyl chain spacer between the silica surface and amide polar group, which gives extra phase stability in eluents of pH 2 - pH 8.  The longer C18 tail of the ligand increases the hydrophobic mechanism of interaction compared to phases with shorter (C14 - C16) tail groups. ACE C18-Amide offers low bleed for UV and LC/MS compatibility and can be used in 100% aqueous mobile phase conditions. Particle sizes of 2, 3, 5 and 10µm are available.

ACE CN-ES combines CN polar selectivity with enhanced hydrophobicity, enabling the benefits of both interactions to be fully exploited. Extra phase stability and increased column lifetime is provided by an extended alkyl chain spacer between the silica surface and cyano group. ACE CN-ES is suitable for both reversed-phase and normal-phase separations and can be used in 100% aqueous mobile phase conditions. Particle sizes of 2, 3, 5 and 10µm are available.

The existing phases in this range, ACE C18-AR and ACE C18-PFP, combine the hydrophobic characteristics of a C18 phase with the selectivity of an aromatic phenyl phase for ACE C18-AR and with the alternative selectivity offered by a pentafluorophenyl phase for ACE C18-PFP. Both of these phases exhibit ultra low bleed to ensure LC/MS compatibility and demonstrate excellent temperature and pH stability. Both phases are also stable in 100% aqueous mobile phase conditions.

The ACE SuperC18 UHPLC and HPLC column range is also manufactured by Advanced Chromatography Technologies Limited. These columns offer excellent stability across an extended pH range (pH 1.5 to 11.5). ACE SuperC18 columns are designed for use with LC/MS compatible buffers and offer ultra-low bleed for improved LC/MS compatibility. They can be used with both MeOH and MeCN mobile phases and offer rapid column equilibration without memory effects.

The full range of ACE Ultra-Inert HPLC and UHPLC columns also includes C18, C8, C4, C18-HL, Phenyl, Cyano, AQ and Silica along with five 300Å wide pore phases (C18, C8, C4, CN and Phenyl).

For further information on the full ACE range of HPLC/UHPLC columns, including unique ACE C18-Amide, ACE CN-ES, ACE C18-AR, ACE C18-PFP and ACE SuperC18, email, or visit the website.