Laboratory Products
The Advantages of Automating Sample Preparation through Supported Liquid Extraction Plates
Author: Elena Gairloch North American Market Manager SPE Consumable Products, Biotage
Free to read
This article has been unlocked and is ready to read.
Download
Sample preparation can be accomplished by
several methodologies including, protein
precipitation (PPT), solid phase extraction
(SPE), and Liquid-liquid extraction (LLE).
In many cases the use of automated liquid
handlers and 96-well plates is desired for
high throughput. When faced with the
challenges of higher performance and
limited resources, developing a robust,
cost effective sample preparation protocol
can be a difficult process.
Now available in the 96-well plate format,
supported liquid extraction (SLE) is
emerging as a preferred technique for
aqueous samples because it is easily
automated, provides clean extracts, method
development is fast and simple, and they
are generally lower cost than most SPE
plates. The 2 step extraction method is
simple to optimise and can be applied
to a wide variety of acidic, basic and
neutral compounds.
This article outlines the SLE technique
compared with traditional LLE for the
application of drugs in human plasma.
Free to read
This article has been unlocked and is ready to read.
Download