Elevating Microscopy to the Next Dimension

Olympus has released the Multichannel 5D Solution module for its cellSens Dimension software. This module will simplify even the most complicated microscopy experiment, by providing advanced, automated acquisition of Z-stacks, multiple fluorescence channels and time-lapse series. Able to seamlessly integrate with other Olympus hardware and software platforms, the new module provides a level of adaptability that will take microscopy studies to the next level.

The Multichannel 5D Solution module from Olympus enables the automated acquisition of images in five dimensions (5D): X, Y, Z, multi-channel with transmission overlay, and timelapse. As a result, this powerful tool can provide researchers with the ability to visualise and analyse multiple features within 3D space as well as monitoring changes over time, thereby enhancing the ability of the cellSens Dimension system to carry out highly customisable and complicated experiments. When used with the automated Extended Focal Imaging (EFI) tool it is possible to obtain perfectly focused images by extracting the sharpest features from each Z-stack before reassembling them into a single, crystal clear image. Furthermore, the EFI tool can be used on-the-fly to display images in real-time.

As well as providing greater physical and temporal flexibility, the new module is ideally suited to performing co-localisation studies by facilitating the spectral unmixing of overlapping fluorescence emission signals. The user interface provides researchers with the ideal workflow, since only the required tools and features are displayed, further simplifying analysis. In combination with the Olympus cellSens Dimension Multiposition module and cellSens Dimension’s built in Multiple Image Alignment (MIA) tool, images from adjacent fields can be flawlessly stitched together to create large panoramas that take advantage of all the features provided by the 5D system.

Furthermore, the process can be automated to achieve a level of complex analysis not possible using manual control. In this way the impressive 5D platform can be elevated into the 6th Dimension, providing users with yet more flexibility and functionality.

Designed to offer control and processing for microscopebased experimental systems, the cellSens Dimension software package provides a broad range of advanced features, as well as specialised, optional Solution modules.