Microscopy & Microtechniques
Easy to Advanced Photo-Manipulation in One New Flexible System
May 19 2010
Olympus has introduced the new advanced cell^FRAP system as part of its newly launched xcellence live cell fluorescence imaging station. Intelligent scanning algorithms that target only the region of interest (ROI) remove the need for line scanning between ROIs and therefore increase the scan speed. With cell^FRAP it is possible to undertake simultaneous photobleaching and image acquisition of multiple ROIs.
cell^FRAP offers pre-defined, easy FRAP experiments for straight forward investigations of cellular dynamics through the seamless integration of FRAP within the ‘Experiment Manager’ software. ‘Fire on Click’ experiments also enable online triggering of single and multi-point bleaching via the mouse for manipulating moving specimens, such as vesicles. For advanced users, high-end photo-manipulation techniques
enabled by the cell^FRAP module include: FLIP, FLAP, photoactivation and photoconversion applications. In the advanced mode users can readily configure the system to their exact requirements. The module also enables pattern bleaching of large numbers of small areas, to simulate Fluorescent Speckle Microscopy (FSM), removing the need for many months of time consuming experiments.
The speed and precision of FRAP delivered by the new Olympus cell^FRAP tool is made possible by using the realtime controller together with camera-based imaging, which is up to 10x faster than point scanner confocal-based FRAP. By providing a complete solution for all photobleaching, photoconversion and photocontrol techniques, cell^FRAP offers far greater value for money than standard confocalbased FRAP.
Furthermore it is fully compatible with the advanced cell^TIRF and cell^SPIN xcellence modules, since it can operate independently of the standard fluorescence and camera ports of the microscope. This means that the system is extremely flexible, as well as fast.
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