Evolving Markets in Raw Milk Consumption Emphasise Need for Routine Testing for E. coli

Following a periodical review of consumer health risks from unpasteurised milk and cream, the Food Standards Agency (FSA) have maintained their recommendation that pasteurisation is an important control measure in reducing the risks from consumption of raw milk. Microbiological data reviewed showed the presence of pathogenic
microorganisms in some raw milk samples and indicators of faecal contamination present at varying levels in most samples analysed. Whilst full results of the review are yet to be published, it is clear that the need for routine testing to detect pathogens such as Escherichia coli as quickly and easily as possible will remain as important as ever.

Lab M’s Modified Lauryl Sulphate Tryptose Broth with MUG & Tryptophan (ISO) is designed specifically for the presumptive enumeration of E. coli from milk and milk products. Used in conjunction with the Most Probable Number (MPN) technique and in accordance with ISO 11866-1:2005, Modified Lauryl Sulphate Tryptose Broth with MUG & Tryptophan (ISO) allows presumptive E. coli to be enumerated from presumptive coliforms. The addition of MUG to the Lauryl Tryptose broth formulation allows the positive discrimination of E. coli strains. The majority of E. coli produce β-glucuronidase enzyme, which enables them to hydrolyse MUG, resulting in the release of a fluorogenic compound. Tubes that fluoresce under UV light are confirmed for E. coli by a positive indole reaction when Kovac’s reagent is  added to the tube.