Chromatography
More and more CD spectroscopists are choosing Chirascan™
Mar 03 2010
With exceptional performance the Chirascan™ range of circular dichrosim spectrometers now enable measurements of biomolecules and their interactions that have not previously been possible.
Optimised solutions for use in biotherapeutic drug development and formulation testing are available giving structural, thermodynamic and aggregation data simultaneously in one hour with as little as 50ug of protein.
By incorporating innovative optical design features to maximise light throughput and intuitive software for data analysis the instruments have outstanding sensitivity and are easy to use.
The rapid acceptance of Chirascan™ is because the instruments offer performance that is simply not available with other CD spectrometers. Superior data can now be obtained easily and quickly with out the need for large amounts of sample. Examples include:
- Thermodynamic, structural and aggregation data in a single experiment with as little as 50µg of protein
- Sample-efficient stopped-flow kinetics with simultaneous CD, absorbance and fluorescence detection
- Genuine, far-UV reach for accurate secondary structure determination
- Low sample-volume near-UV measurements for insights into tertiary structural behaviour
Chirascan™-Plus DMS is the most advanced instrument in the range and is an optimised solution for use in biotherapeutic drug development. In a recent formulation study of a biotherapeutic antibody the technology was not only used to identify melting point transitions as an indicator of stability but also enabled the propensity for aggregation to be taken into consideration when making decisions.
A stopped flow accessory can be coupled to Chirascan™ instruments for studying how the structure of a biomolecule changes following interaction with another molecular entity or to study refolding mechanisms on dilution of a chemical denaturant. Simultaneous measurement of absorbance, fluorescence and circular dichroism maximises the information return on the sample investment.
AppliedPhotophysics
Find out more www.photophysics.com
Digital Edition
ILM 49.5 July
July 2024
Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...
View all digital editions
Events
Jul 30 2024 Jakarta, Indonesia
Jul 31 2024 Chengdu, China
ACS National Meeting - Fall 2024
Aug 18 2024 Denver, CO, USA
Aug 25 2024 Copenhagen, Denmark
Aug 28 2024 Phnom Penh, Cambodia