Microscopy & Microtechniques
DEVELOPMENT OF A TWO PHOTON BURST INTEGRATED FLUORESCENCE LIFETIME SYSTEM FOR SINGLE-PAIR FRET TO ANALYSE INTERACTING PROTEIN POPULATIONS
Nov 05 2008
Author: Senthila Pathmananthan James Hunt Daniel R. Matthews Andrew J. Beavil Tony Ng Simon M. Ameer-Beg
Fluorescence detection of single molecules
represents the ultimate in sensitivity for
fluorescence-based assays in biology and
medicine by eliminating ensemble-averaging.
We describe the development of a time-resolved
burst integrated fluorescence lifetime (BiFL)
methodology to observe single-molecule
dynamics and measure fluorescence lifetime
of these molecules as they diffuse through
a femtoliter volume probed by two
photon excitation.
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