Chromatography
HPLC Assay of Water-Soluble Vitamins, Fat-Soluble Vitamins, and a Preservative in Dry Syrup Multivitamin Formulation - Suparerk Tukkeeree and Jeffrey Rohrer
Nov 04 2010
Author: Suparerk Tukkeeree and Jeffrey Rohrer on behalf of Thermo Fisher Scientific Chromatography and Mass Spectrometry
INTRODUCTION
Vitamins are vital to human development and long-term health; therefore, infants are usually prescribed a vitamin supplement to ensure they receive the recommended daily allowance of each vitamin. Children under one year of age are usually given this supplement in liquid form. This supplement can be produced as a dry syrup using a powdered preparation to which the pharmacist adds liquid to produce the
dosage form for the patient. The work shown here describes an HPLC method to quantify water- and fat-soluble vitamins in a dry syrup.
Vitamins were extracted from the dry syrup prior to analysis. The water soluble vitamins (WSV) were extracted with water and a pH adjustment with KOH to dissolve folic acid. The fat soluble vitamins (FSV) were extracted with either DMSO or ethyl acetate. To include all vitamins in the same chromatogram, the authors used a Chromeleon® Chromatography Data System (CDS) software feature that
allows more than one injection for the same analysis. The WSV sample was injected first, then after elution of all WSV, the FSV sample was injected. This application also can be run by UHPLC using a 2.2 μm Acclaim PA2 column in 2.1 × 100mm format to save time, reduce mobile phase consumption, and reduce waste. This document shows that the UltiMate® 3000 system with an Acclaim PA2 column is
an excellent solution for vitamin determinations.
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